The NCAA has officially revised its gender eligibility policies, barring biological males from competing in women’s sports. This decision was made public just one day after President Donald Trump signed an executive order aimed at addressing gender-based participation in athletics.

On Thursday afternoon, the NCAA, which oversees college sports nationwide, announced its updated policy on transgender student-athletes. According to the new regulations, “A student-athlete assigned male at birth may not compete on a women’s team.” However, biological females will still be permitted to compete on men’s teams.

Previously, under a policy in place since 2010, transgender athletes were allowed to participate in women’s sports after undergoing at least one year of testosterone suppression therapy.

Trump signed the “No Men in Women’s Sports” executive order at the White House on Wednesday, coinciding with National Girls and Women in Sports Day. In response, NCAA President Charlie Baker issued a statement acknowledging the executive order, describing it as a “clear, national standard.” He indicated that the NCAA Board of Governors would review the policy and work to align with the administration’s directive.

“The NCAA is an organization made up of 1,100 colleges and universities in all 50 states that collectively enroll more than 530,000 student-athletes,” Baker stated. “We strongly believe that clear, consistent, and uniform eligibility standards would best serve today’s student-athletes instead of a patchwork of conflicting state laws and court decisions. To that end, President Trump’s order provides a clear, national standard.”

The NCAA has officially changed their 'transgender participation' policy effective immediately.

I can't even begin to tell you how vindicating it feels knowing no girl will ever have to experience what my teammates and I did.

Thank God Trump is back in office. pic.twitter.com/2USfSuZ6ij

— Riley Gaines (@Riley_Gaines_) February 6, 2025

Baker further noted that the NCAA would take “necessary steps to align NCAA policy in the coming days, subject to further guidance from the administration.” He reassured institutions that the NCAA remains committed to fostering “welcoming environments on campuses for all student-athletes” while providing support for those affected by the policy change.

The issue of transgender participation in women’s sports has sparked significant debate and legal battles. During a Senate Judiciary Committee hearing in December, Baker addressed concerns about female athletes being required to share teams and locker rooms with transgender athletes. He defended the previous policy, citing federal standards, but acknowledged that female athletes uncomfortable with such arrangements had the option to seek alternative accommodations.

Multiple lawsuits have been filed against the NCAA and its member schools over the participation of biological males in women’s sports. Former NCAA swimmer and conservative activist Riley Gaines is leading a lawsuit stemming from her experience competing against and sharing a locker room with transgender swimmer Lia Thomas at the 2022 national championships. Several other female athletes have joined her legal challenge.

On Tuesday, three former University of Pennsylvania swimmers, who were teammates of Thomas, also filed a lawsuit. They alleged that university administrators dismissed their concerns and pressured them to accept policies that allowed Thomas to compete against women.

Meanwhile, the Department of Education (DOE) has launched investigations into potential Title IX violations related to transgender participation in women’s sports. Cases under review include complaints against the University of Pennsylvania, San Jose State University, and the Massachusetts Interscholastic Athletic Association (MIAA).

At San Jose State, transgender volleyball player Blaire Fleming played three seasons on the women’s team from 2022 to 2024, becoming one of the Mountain West Conference’s top hitters. Unlike UPenn’s handling of Thomas, administrators at SJSU allegedly did not inform female athletes that Fleming was transgender. This has led to a lawsuit filed by 11 Mountain West volleyball players and a former assistant coach, who claim the university misled them.

Sources told Fox News Digital that the DOE’s Office for Civil Rights is now reviewing athletic participation policies across various schools to assess their compliance with Title IX protections for female athletes. Last week, the DOE officially reinstated enforcement of Title IX protections based on biological sex, impacting K-12 schools and higher education institutions nationwide.